Tsukuba City Japan, August 3, 2023 /PRNewswire/ — Scientists at the Materials Nanoarchitectonics Research Center (MANA) have developed micrometer-sized “liquid marbles” that can encapsulate from a single cell to several living cells, thereby creating a single Assisted with cell research.
To analyze individual cells, the required number of cells must be spatially separated. This often requires extensive training and the use of expensive laboratory equipment. Scientists at MANA recently used nanotechnology to overcome this hurdle. They coated microdroplets of cell suspensions with superhydrophobic nanoparticles (small particles with surfaces with exceptional water-repellent properties) and developed customizable sizes ranging from one to hundreds of micrometers. Spherical structures known as “dry cells” were created. Easy to pick up. This facilitates cell selection and greatly facilitates single-cell analysis.
To accomplish this, the research team manually sprayed suspended cells to create a “mist” of cells. Upon contact with the vibrating nanoparticle bed, the cell-laden mist droplets are instantly coated with superhydrophobic fumed silica nanoparticles, resulting in a liquid marbled dry cell with a dry powdery outer layer surrounding an inner liquid core. generated. In fact, scientists have been able to create dry batteries with an internal water content of 95% or more, surrounded by a dry outer layer. These droplets exhibited a powder-like smooth flow and could be easily picked up with tweezers without being damaged or spilling the liquid inside. Furthermore, superhydrophobic silica nanoparticles within the surface coating prevented droplet coalescence.
Scientists were able to sort the batteries according to size by sieving them. We were also able to simultaneously encapsulate normal and cancerous cells and create several cell colonies within these dry cells. In addition, we were able to easily introduce liquids into and remove liquids from these droplets. Finally, they observed that the nanoparticles could be separated from the cell suspension layer by centrifugation, and found that this made the process recyclable.
“Our dry cell manufacturing method efficiently facilitates cell selection, lowers the barriers for single-cell research, and greatly increases the accessibility and productivity of single-cell analysis,” concludes Dr. I’m here. Koichiro UtoOn behalf of my co-author, Mizuki Tenjinbayashi, Shota Yamamoto.
Research Highlights Vol. 83
MANA Research Highlights
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SOURCE National Institute for Materials Science (NIMS) Center for Materials Nanoarchitectonics (MANA)